中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5401-5406.doi: 10.3969/j.issn. 2095-4344.2012.29. 017

• 药物控释材料 drug delivery materials • 上一篇    下一篇

胶质细胞源性神经营养因子缓释微球及NogoA、硫酸软骨素酶ABC缓释微球联合促大鼠损伤脊髓再生神经功能的修复

张 宇1, 2，宋跃明1   

  1. 1四川大学华西医院骨科，四川省成都市 610041；
    2成都市第一人民医院骨科，四川省成都市 610021
  • 收稿日期:2012-03-15 修回日期:2012-04-20 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 宋跃明，教授，博士导师，四川大学华西医院骨科，四川省成都市 610041 sym-cd@yahoo.com.cn
  • 作者简介:张宇☆，男，1978年生，四川省成都市人，汉族，2009年四川大学毕业，博士，主治医师，主要从事脊柱外科研究。 yuxli@cisco.com

Effect of the combination of glial cell line-derived neurotrophic factor, NogoA and chondroitin sulfate ABC microspheres on promoting the neural regeneration of rat’s injured spinal cord

Zhang Yu1,2, Song Yue-ming1   

  1. 1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;
    2Department of Orthopedics, Chengdu First People’s Hospital, Chengdu 610021, Sichuan Province, China
  • Received:2012-03-15 Revised:2012-04-20 Online:2012-07-15 Published:2012-07-15
  • Contact: Song Yue-ming, Professor, Doctoral supervisor, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China yuxli@cisco.com
  • About author:Zhang Yu☆, Doctor, Attending physician, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; Department of Orthopedics, Chengdu First People’s Hospital, Chengdu 610021, Sichuan Province, China yuxli@cisco.com

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482

被引次数

4

摘要：

背景：前期研究证实胶质细胞源性神经营养因子促损伤脊髓再生修复效果良好。
目的：观察胶质细胞源性神经营养因子、NogoA与硫酸软骨素ABC缓释微球联合促进大鼠损伤脊髓再生运动功能修复的作用。
方法：取64只SD大鼠随机分为正常对照组、假手术组(仅行椎板切除)、模型组、胶质细胞源性神经营养因子组、胶质细胞源性神经营养因子微球组、硫酸软骨素ABC微球组、NogoA抗体微球组、联合组(胶质细胞源性神经营养因子微球+硫酸软骨素ABC微球+NogoA抗体微球)。后6组制备T10脊髓完全横断伤模型，于脊髓断端间缓慢注射一半相应药液，另用一块明胶海绵吸附另一半相应药液覆盖断端背面。
结果与结论：①BBB 运动功能评分：术后5，6，7，8，9，10周，联合组评分高于模型组、胶质细胞源性神经营养因子组、胶质细胞源性神经营养因子微球组、硫酸软骨素ABC微球组、NogoA 抗体微球组(P < 0.05)。②术后10周皮质体感诱发电位：假手术、模型组、胶质细胞源性神经营养因子组均未检测出。联合组主反应潜伏期低于其他各组(P < 0.05)，但高于正常对照组(P < 0.05)；主反应峰峰值高于其他各组(P < 0.05)，但低于正常对照组(P < 0.05)。说明胶质细胞源性神经营养因子缓释微球及NogoA、硫酸软骨素ABC 缓释微球联合促大鼠损伤脊髓再生神经功能修复效果优于单用胶质细胞源性神经营养因子缓释微球。

关键词: 胶质细胞源性神经营养因子, 微球, 聚乳酸-聚羟基乙酸共聚物, 脊髓损伤, 神经再生

Abstract:

BACKGROUND: Previous studies have shown that glial cell line-derived neurotrophic factor (GDNF) exhibits good effect on the regeneration of the injured spinal cord.
OBJECTIVE: To investigate the effect of the combination of the injured chondroitin sulfate ABC (ChABC), GDNF and NogoA microspheres on the regeneration and functional recovery of the injured spinal cord in rats.
METHODS: Totally 64 adult SD female rats were randomly divided into eight groups: normal control group, sham operation group (only laminectomy), model group, GDNF group, ChABC microspheres group, GDNF microspheres group, NogoA microspheres group, ChABC+GDNF+NogoA microspheres group. T10 total transected spinal cord models were established in all groups except for normal control and sham operation groups, and the broken ends of the injured spinal cord were injected a half of liquid medicine, and then the back of the broken ends were covered by the other half of liquid medicine which was absorbed by latin sponge.
RESULTS AND CONCLUSION: ①Basso, Beattie and Bresnahan (BBB) Locomotor Rating scores: ChABC+GDNF +NogoA microspheres group was higher than the model, GDNF, GDNF microspheres, ChABC and NogoA microspheres groups at weeks 5, 6, 7, 8, 9 and 10 after treatment (P < 0.05). ②Cortical somatosensory evoked potential (CSEP) at week 10 after treatment: no detection was found in the sham operation , model and GDNF groups. CSEP in main reaction latency of the ChABC+GDNF+NogoA microspheres group was lower than that of other groups (P < 0.05), but higher than that of the normal control group (P < 0.05). The peak value of the main reaction peak in the ChABC+GDNF+NogoA microspheres group was higher than that in other groups (P < 0.05), but lower than that in the normal control group (P < 0.05). These findings suggest that the combination of GDNF, NogoA and ChABC microspheres can promote the regeneration, functional recovery and nerve conduction of the spinal cord in rats, which is superior to that of pure GDNF microspheres.

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